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Publication Date: Mon Mar 29 00:00: Research Org.: Lawrence Livermore National Lab. Northwestern Univ., Evanston, IL (United States).Ludwig-Maximilians-Univ., München (Germany).of California, San Francisco, CA (United States) Johannes Gutenberg Univ., Mainz (Germany).Pennsylvania State Univ., University Park, PA (United States).Georgia Institute of Technology, Atlanta, GA (United States).of California, Los Angeles, CA (United States)
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The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures.
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Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics.